Chemical, Biological and Microbiological Studies on the Seeds and Peels of Annona Fruit

Abstract

The current investigation was carried out to examine the possible potential protective effects of ethanolic extracts of different parts of Annona fruit against trichloroacetic acid induced liver cancer. The chemical composition of the seeds and peel of Annona fruit were evaluated. After drying the seeds and peel, the bacterial, fungal and yeast count for a month. The total content of flavonoids, phenols, and antioxidants was estimated using DPPH, and HPLC conditions of phenols of all extracts. The biological experiment used thirty six albino rats. After the adaptation period, the rats were divided into six groups (6 rats each). One of them was considered as a negative control and another positive control (Trichloroacetic acid group). The remaining groups were divided into four groups consisting of extracts from seeds and peel at levels 100 and 150 mg / kg. Blood samples were collected to assays the levels of liver functions (ALT, AST, ALP, BIL, TP, ALB and GLB), some kidney functions (creatinine and urea), and oxidation stress (CAT, GPX and MDA). The study results were as the fallowing: all liver cancer protected groups marked a significant improvement in liver functions, some kidney functions, and oxidation stress, when compared them with the unprotected liver cancer group (+ve). Therefore, this study recommends that the insertion seeds and peels of Annona fruit in diets because it has anticancer and antioxidants effects.