The Influence of Titanium Dioxide Nanoparticles on the Ultrastructure of Bacterial and Fungal Cells, And Evaluation of Their Cytotoxicity


  • Nanis G. Allam Microbiology Unit, Botany Department Faculty of Science, Tanta University, Egypt.
  • Mohammed M. Gharieb Botany and Microbiology Department, Faculty of Science, Menoufia University, Egypt.
  • Noha S. El-Nahrawy Microbiology Unit, Botany Department Faculty of Science, Tanta University, Egypt.


Antimicrobial activity, Transmission electron microscopy, Cytotoxicity assay, WST-1 assay, IC50


The nanoparticles of titanium dioxide (Tio2NPs) are among the most promising nanomaterials which widely used at the commercial level, especially in cosmetics, medicine and food industry. In our study, the antimicrobial effect of titanium dioxide nanoparticles on pathogenic micro-organisms was explained by transmission electron microscopy, which was used to investigate the mode of action of TiO2NPs on bacterial and fungal cells. Staphylococcus aureus, Bacillus cereus, Salmonella spp. and Penecillium spp. were greatly affected by TiO2NPs. The cytotoxicity assay of Oral Epithelial cell (OEC) lines was studied using water soluble tetrazolium salt (WST-1 assay) for TiO2 nanoparticles at different concentrations 0.01 mg/ml, 0.1 mg/ml, 0.3 mg/ml, 1 mg/ml and 10 mg/ml. The cell viability of the oral epithelial cell lines were 96.26 %, 83.16 %, 81.82 %, 76.94 % and 55.8 % respectively, and IC50 of TiO2NPs was greater than 10 mg/ml concentration. The obtained results recommended the non-toxic nature of titanium dioxide nanoparticles and their antimicrobial activity against different food borne pathogens that would has a potentiality in the context of food preservation.