Hepatoprotective Impact of Vitamin C against Mitochondrial Dysfunction Induced by Malathion on Rat Liver

Authors

  • Mona Saber Hamed Biochemistry Department, Egyptian Drug Authority, Formerly Known as National Organization for Drug Control and Research, Egypt.
  • Sawsan Ahmed Nasr Physiology Department, Egyptian Drug Authority, Formerly Known as National Organization for Drug Control and Research, Egypt.
  • Sanaa M. Abdulrahman Central Agricultural of pesticides Lab., Agriculture Research Center, Egypt.

Keywords:

Nrf2, Keap1, histology, Malathion, hazardous

Abstract

The present study is designed to investigate the possible hepatoprotective effect of vitamin C on liver of rats. A total number of 32 female albino rats. Animals were divided into four groups; Group 1: Negative control group Group 2: Positive control group, animals in this group were administrated with Malathion (50 mg/kg/day) intraperitoneally, Group 3: Animals in this group were orally administrated with vitamin C (100 mg/kg/day) and Group 4: Animals in this group were  administrated with malathion (50 mg/kg/day) intraperitoneally with vitamin C (100 mg/kg/day) orally. The blood samples were centrifuged in cooling centrifuge to separate serum to measure aspartate amino transferase (AST) and alanine amino transferase (ALT) activity, total protein and albumin. Tissues of the liver was homogenized to estimate the content of reduced glutathione (GSH), malondialdehyde (MDA) and the activity of copper-zinc superoxide dismutase (SOD). Also another portion of the liver tissue was homogenized to isolate mitochondria to estimate the content of GSH, MDA and the activity of SOD, nuclear factor-erythroid 2-related factor2 (Nrf2) and kelch like ECH associated protein 1(keap1). Histological examination of the tissues was conducted after removal of liver tissues from rats. The results indicated a significant increase MDA level in the liver and mitochondria homogenates of malathion-treated rats compared to the negative control ones. Malathion also resulted in a significant decrease in liver and mitochondrial GSH content. ALT & AST activities significantly increased while protein and albumin significantly decreased compared to the negative control. The obtained results illustrated that rats treated with Malathion for 30 days showed a significant decrease in Nrf2 while Keap1 decreased significantly compared to the negative control
group. Animals treated with malathion+ vitamin C showed an improvement in all these parameters and the histological study  confirmed these result. In a conclusion, vitamin C can ameliorate the  hazardous effects of Malathion.

Published

2022-01-05