The Effect of Treatment on HIF-1 Alpha Gene Expression, Cell Growth and Apoptosis in Breast Cancer MCF-7 Cell Line

Abstract

Background and Objectives: Breast cancer is the most frequent cancer in women globally, the second greatest cause of cancer mortality in women after lung cancer, and the fifth highest cause of death from cancer illnesses overall. Per the World Health Organization (WHO) estimates from 2012, accounting for 23% of all cancer deaths. Breast Cancer was the top tumor among women in all cancer incidences in the Middle East, accounting for 27.7 percent to 38.2 percent of all recorded tumors. Breast cancer is mostly caused by hereditary mutations in the BRCA1 and BRCA2 breast cancer susceptibility genes. Methodology: To achieve this aim we conducted the study on MCF-7 cell lines and evaluated the cytotoxicity of different concentration of Curcuma Longa Extract, on the expression of genes (HIF-1α, HIF-1β, BAX and BCL-2. Results: The results showed that the different concentration of Curcuma Longa Extract inhibited the proliferation of the Breast cancer cells (MCF- 7) through upregulation Pro-Apoptotic gene BAX and downregulation of gene Anti- Apoptotic BCL- 2 and also downregulate HIF-1α and HIF-1β as a result cancer pathways have been arrested. we investigated the ability of Curcuma Longa Extract to inhibit MCF-7 cell lines growth by cytotoxicity assay after the MCF-7cell lines were treated with various concentrations of Curcuma Longa Extract (25-50 μM) for 24 and 48h. In the viability of MCF-7cell lines for 24 and 48 h. All concentrations were able to inhibit the proliferation of the cancer cells.